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The effect of temperature and pH on psychrophilic chitinase expression in Pichia pastoris X-33

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Title
The effect of temperature and pH on psychrophilic chitinase expression in Pichia pastoris X-33
Other Titles
재조합 효모에 의한 저온성 chitinase의 발현에 미치는 온도 및 pH의 영향
Authors
Han, Se Jong
Park, Heeyong
Yim, Joung Han
Koh, Hye Yeon
Lee, Sung Gu
Keywords
AntarcticPichiachitinasepsychrophilic
Issue Date
2009
Citation
Han, Se Jong, et al. 2009. The effect of temperature and pH on psychrophilic chitinase expression in Pichia pastoris X-33. (사) 한국생물공학회. (사) 한국생물공학회. 2009.11.02~.
Abstract
To confirm the optimal cultivation conditions of Pichia pastoris X-33 containing the psychrophilic chitinase gene cloned from Antarctic microorganism, Sanguibacter antarcticus KOPRI 21702, the effect of temperature and pH on chitinase expression was investigated in a jar fermentor. Expression of target protein was performed by methanol addition and confirmed by an activity assay with p-nitrophenyl-N-acetyl-
-D-glucosaminide as a substrate. Cells were grown at various temperatures ranging from 20 to 30 
C and pHs from 5.5 to 6.5. The chitinase production was divided into two phases: batch and induction fed-batch. Ultimately, the optimal temperature and pH for the production of chitinase were found to be 20 
C, pH 6.0, respectively.
URI
https://repository.kopri.re.kr/handle/201206/7962
Conference Name
(사) 한국생물공학회
Conference Place
(사) 한국생물공학회
Conference Date
2009.11.02~
Type
Proceeding
Indexed
Pro(초록)국내
Appears in Collections  
2006-2010, Procurement and utilization of polar genetic resources (06-10) / Lee, Hong Kum; Yim, Joung Han (PE06050, PE07050, PE08050, PE09050, PE10050)
2009-2010, 극지 미생물 유래 재조합 단백질 생산 시스템 개발 (09-10) / Han, Se Jong (PK09040)
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