Medium optimization of chitinase production from the Antarctic bacterium, Sanguibacter antarcticus KOPRI 21702 using statistical experimental design
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- Medium optimization of chitinase production from the Antarctic bacterium, Sanguibacter antarcticus KOPRI 21702 using statistical experimental design
- Other Titles
- 남극 미생물 상귀박터 안탁티쿠스 KOPRI 21702로 부터 chitinase를 생산할때 통계적방법을 적용한 배지최적화
- Lee, Sung Gu
Yim, Joung Han
Lee, Hong Kum
Han, Se Jong
- Antarctic bacterium; psychrophilic chitinase; statistical optimization
- Issue Date
- Lee, Sung Gu, et al. 2010. Medium optimization of chitinase production from the Antarctic bacterium, Sanguibacter antarcticus KOPRI 21702 using statistical experimental design. 한국생물공학회. 한국생물공학회. 2010.04.16~.
- The medium components were optimized by using a statistical experimental design approach for increasing chitinase production from the Antarctic bacterium Sanguibacter antarcticus KOPRI 21702. To increase the production of psychrophilic chitinase, significant carbon and nitrogen sources were determined and other elements were chosen by using a two-level Plackett-Burman statistical design technique. In the first optimization step, glycerol and peptone were selected as main carbon and nitrogen sources, respectively. In the second step, the influence of fifteen components included in Marine Broth medium on chitinase production was evaluated. As a result, NaCl, Fe(C6H5O7), MgCl2, Na2SO4, H3BO3, and CaCl2 were found to have a positive influence on the chitinase production, while KCl, KBr, and NH4NO3 had a negative influence. In the third step, the concentrations of NaCl, Fe(C6H5O7) and MgCl2 were further optimized. The maximum chitinase activity obtained was 89 U/L using 10 g/L glycerol, 5 g/L peptone, 23 g/L NaCl, 0.01 g/L Fe(C6H5O7), and 2.5 g/L MgCl2. [This work was supported by a grant from the Korea Polar Research Institute (PE10050).]
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